研究主題

Profiling Analysis of Isomalto-Oligosaccharides Using LC-MS/MS
 

研究摘要

Isomaltooligosaccharides are indigestible carbohydrates with prebiotic properties. Analytical platforms are important for the food industry to monitor the characteristics and to study the properties of isomaltooligosaccharides. However, the profile of isomaltooligosaccharides is complex with a versatile polymerization number of sugar units and linkage types, which presents a challenge, especially identification of isomers. The objective of this study is to use liquid chromatography-tandem mass spectrometry (LC-MS/MS) to establish an analytical platform for determining the profiles of complex isomaltooligosaccharides. Graphitized carbon liquid chromatography (GCC) of LC was used to resolve complex isomers. The developed platform can identify the isomers from degree of polymerization (DP) 2 to 12 by the elucidation of the characteristic fragment ions. Dimers, trimers, and tetramers are major components of selected commercial isomaltooligosaccharides products. Six dimers, 13 trimers, and 10 tetramers were identified. α-1→6 and 1→4 were the major linkages, whereas isomers with α-1→2, 1→3, and even 1?1 linkages were also identified. The DP proportions of 3 commercial isomaltooligosaccharides products were similar while isomer profiles of dimers, trimers, and tetramers were significantly different. The major proportion differences contributed from 62 glucosyl maltose, 63 glucosyl maltotriose, isomaltotetraose. The established analytical platform can be a useful tool for studying the structures and the prebiotic-efficacy relationship of isomaltooligosaccharides products.
 

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